Coding

Part:BBa_K1155009:Design

Designed by: LE LAM Thao Nguyen, Xiaojing, Damir, Nadia   Group: iGEM13_Paris_Saclay   (2013-09-19)


bphR2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 277


Design Notes

Change site of PstI in coding sequence from CTGCAG (Leu,Gln) to CTACAG (Leu, Gln)


Source

Pseudomonas pseudoalcaligenes KF707 genomic DNA

References